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Registros recuperados: 170
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - First Periodic Progress Report 4th January 1999 to 3rd January 2000 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure. 2 - Comparing oyster herpes-like viruses with other viruses belonging to the Helpesviridae family on the basis of sequence data and...
Tipo: Text Palavras-chave: Pathology; Diagnosis; Oyster; Herpes like virus; Herpesviridae; Immunology; Detection; Virus; Bivalves.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00044/15523/12910.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" Third Periodic Progress Report : 4th January 2001 to 3rd January 2002 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Herpesvirus infections have been reported in different bivalves species around the world. Specific diagnostic tools are needed in order ta assess the causative raIe of these agents in mortalities observed among bivalves There is currently a lack of information concerning the occurrence of bivalve herpesviruses. This is probably due ta the lack of suitable diagnostic tools. The basic method for examination of bivalve samples is predominantly histology. This enables the identification of any cellular changes, but is not conclusive for virus identification. Transmission electron microscopy is a necessity for visual confirmation of viral infections. However, histology and electron microscopy are time consuming and inadequate for epidemiological studies. The...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Virus; Oyster; Detection; OsHV-1; Herpesviridae.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00044/15524/12911.pdf
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Diagnostic moléculaire de l'origine des contaminations fécales dans l'environnement littoral - Développement de marqueurs Bacteroidales spécifiques de l'hôte ArchiMer
Mieszkin, Sophie.
Human and animal faecal pollution affects environmental water in inland and coastal areas, with negative implications for recreational uses, public safety and shellfish sanitary status due to the presence of enteric pathogens. Starting from 2011, the revised Bathing Water European Directive (2006/7/CE) requires the establishment of bathing water profiles with an inventory and study of the pollution sources likely to affect water quality. The faecal microbiological indicators used in these regulations, Escherichia coli and enteroccoci, cannot distinguish between human and animal faecal contamination. Thus, alternative methods, that focus on target microorganisms such as bacteria belonging to the Bacteroidales order were developed or are being developed to...
Tipo: Text Palavras-chave: Contaminations fécales humaines; Porcines et ruminants; Bacteroidales; Gènes codant les ARNr 16S; PCR quantitative en temps réel; Eaux; Persistance; Huîtres; Human; Porcine and ruminant faecal contaminations; Bacteroidales; 16S rRNA genes; Quantitative real-time PCR; Water; Persistence; Oyster.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00021/13206/10258.pdf
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Differential Mortality and High Viral Load in Naive Pacific Oyster Families Exposed to OsHV-1 Suggests Tolerance Rather than Resistance to Infection ArchiMer
Agnew, M. Victoria; Friedman, Carolyn S.; Langdon, Christopher; Divilov, Konstantin; Schoolfield, Blaine; Morga, Benjamin; Degremont, Lionel; Dhar, Arun K.; Kirkland, Peter; Dumbauld, Brett; Burge, Colleen A..
Pacific oysters, Crassostrea gigas, are one of the most productive aquaculture species in the world. However, they are threatened by the spread of Ostreid herpesvirus-1 (OsHV-1) and its microvariants (collectively “µvars”), which cause mass mortalities in all life stages of Pacific oysters globally. Breeding programs have been successful in reducing mortality due to OsHV-1 variants following viral outbreaks; however, an OsHV-1-resistant oyster line does not yet exist in the United States (US), and it is unknown how OsHV-1 µvars will affect US oyster populations compared to the current variant, which is similar to the OsHV-1 reference, found in Tomales Bay, CA. The goals of this study were to investigate the resistance of C. gigas juveniles produced by the...
Tipo: Text Palavras-chave: OsHV-1; Mu vars; Oyster; Virus; Tolerance; Virulence; QPCR.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00665/77739/79862.pdf
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Du concept de santé à la détermination d’intervalles de référence chez l’huître creuse Crassostrea gigas ArchiMer
Francois, Cyrille.
Of interest of everyone, health is not an easy concept to figure out. Although there is no universal definition of health, its various uses and meanings allow us to specify its biological, psychic and social dimensions. In animal health, the biological dimension remains the most studied, there is therefore no consensual definition of international animal health. So how to understand the health of marine shellfish? A living being can be functionally described in physiopathology by monitoring biomarkers under laboratory conditions. In men and many animals, "normal" biomarker values have been proposed; these have been determined by carrying out measurements or observations in samples of healthy populations, the rigorous selection of these populations and...
Tipo: Text Palavras-chave: Santé; Huître; Valeurs de référence; Filtration; Consommation d’oxygène; Health; Oyster; Reference values; Clearance rate; Oxygen consumption rate.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00610/72189/70970.pdf
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Dynamique des sédiments fins et mixtes des zones intertidales de la baie de Marennes-Oléron ArchiMer
Kervella, Stephane.
This thesis aims at increasing the general knowledge on sediment dynamics of intertidal flats in the Marennes-Oléron Bay, according to physical, biological and anthropogenic factors. The conducted research is compound of three complementary studies: (1) a study of sediment behaviour through applied rheophysics on intertidal superficial sediments; (2) a hydro-sedimentary study based on identification of the forcing action (physical, biological or anthropogenic) on the intertidal flat stability over several time scales (from the event to several months); (3) a specific application of the model SiAM-2DH to the Marennes-Oléron Bay. The surface sediments shows a mechanical behaviour that mainly depends on the fine fraction for a composition that contains up...
Tipo: Text Palavras-chave: Rhéophysique; Seuil d’écoulement; Sédiments cohésifs et mixtes; Sédimentation; Érosion; Hydrodynamique; Vasière; Zone intertidale; Conchyliculture; ALTUS; SiAM-2DH; Baie de Marennes-Oléron; Rhéophysics; Shear strenght; Cohesive and mixed sediment; Sédimentation; Erosion; Hydrodynamics; Mudflat; Intertidal area; Oyster; ALTUS; SiAM-2DH; Marennes-Oléron Bay..
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00004/11534/8117.pdf
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Écologie de la reproduction de l’huître Crassostrea gigas en lagune méditerranéenne ArchiMer
Lagarde, Franck.
The Mediterranean lagoons host many anthropogenic uses including shellfish aquaculture since the beginning of the 20th century. Imported in 1970, the cupped oyster, Crassostrea gigas, has adapted perfectly and has allowed an important development of oyster’s industry until 2008, the year of occurence of spat outbreak related to the emergence of an Ostreid Herpes-virus μvar. The scarcity of spat and the speculation on this product changed regionally cultural practices and the supply of juveniles, with in particular the wish of developing a local activity of spat collection. However the cycle of reproduction of the species and the recruitment of juveniles were poorly known in lagoon environments. In this context, the objective of this thesis is to...
Tipo: Text Palavras-chave: Huître; Écologie larvaire; Ressource trophique; Connectivité; Recrutement; Lagune côtière; Oyster; Larval ecology; Trophic resource; Connectivity; Recruitment; Coastal lagoon.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00472/58347/60901.pdf
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Economic Analysis of Using Soybean Meal as a Mushroom Growing Substrate AgEcon
Fewell, Jason E.; Gustafson, Cole R..
Mushrooms have been grown commercially on many different substrates for years, usually agricultural by-products such as straw or stover. Increased popularity for specialty mushrooms with consumers has led to increased production and great demand for economic substrates. Oyster mushrooms are easier to grow relative to other types of mushrooms and their production has increased dramatically in recent years. This study examines the economic feasibility of using soybean hulls as a primary substrate for oyster mushrooms, replacing traditional wheat straw. The study uses a cost-benefit analysis to determine an optimal substrate based on yield and the number of crops harvested per year. The study shows that soybean hulls, combined with corn gluten or...
Tipo: Report Palavras-chave: Oyster; Mushrooms; Substrate; Soybean; Hulls; Meal; Economic; Feasibility; Crop Production/Industries.
Ano: 2007 URL: http://purl.umn.edu/7634
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Ecophysiological and metabolic adaptations to sulphide exposure of the oyster Crassostrea gigas ArchiMer
Le Moullac, Gilles; Cheize, Marie; Gastineau, Olivier; Daniel, Jean-yves; Le Coz, Jean-rene; Huvet, Arnaud; Moal, Jeanne; Pouvreau, Stephane; Van Wormhoudt, A; Samain, Jean-francois.
The purpose of this study is to examine the response of the oyster Crassostrea gigas to sulphide at different biological scales. A first experiment was designed to measure for 20 h the clearance (CR) and oxygen consumption (OC) rates of oysters exposed at different concentrations of sulphide (0, 3, 6, 12, 20, and 40 mu M). The second experiment was carried out to evaluate the metabolic adaptations to chronic sulphide exposure for 10 days by measuring PK and PEPCK enzyme activities, succinate and alanine content, the adenylate energy charge (AEC), and the activity of the electron transport system (ETS)in whole oysters at above 20 mu M. PK and PEPCK mRNAs and enzyme activities have been measured in adductor muscle. When exposed to sulphide above 20 mu M, CR...
Tipo: Text Palavras-chave: ATP; AEC; Electron transport system; Succcinate; Alanine; Phosphoenolpyruvate carboxykinase; Pyruvate kinase; Oxygen consumption; Clearance rate; Crassostrea gigas; Sulphide; Oyster.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3924.pdf
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Ecophysiological study on the Pacific oyster Crassostrea gigas naturally infected by a Chlamydia-like microorganism : effect of infection level and diet on oyster physiological responses ArchiMer
Soletchnik, Patrick; Goulletquer, Philippe; Cochennec, Nathalie; Renault, Tristan; Geairon, Philippe.
A population of Pacific ovsters, Crassostrea gigas, infected with a Chlamydia-like procaryote was reared to compare infection trends under three different feeding conditions: a starved condition, an intermediate condition with a diet of Skeletonema costatllm, and a mixed algal diet. Several physiological and pathological criteriJ: presence of gill abnormality; microscopic examination, basophilic intracytoplasmic inclusions, Feulgen reaction; condition index, filtration, food consumptiQn; fecal production and respiration were used to compare the various modalities one month after the initiation of the experiment. The Irodality of starved oysters showed reduce physiological performances in terms of scope for growth and condition index compared to oysters...
Tipo: Text Palavras-chave: Pathogen; Chlamydia; Procaryote; Crassostrea gigas; Oyster; Ecophysiology; Pathogène; Chlamydia; Procaryote; Crassostrea gigas; Huître; Ecophysiologie.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-2741.pdf
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Ecophysiologie de la maturation sexuelle et de la ponte de l'huître creuse Crassostrea gigas : réponses métaboliques (respiration) et alimentaires (filtration, absorption) en fonction des différents stades de maturation ArchiMer
Soletchnik, Patrick; Razet, Daniel; Geairon, Philippe; Faury, Nicole; Goulletquer, Philippe.
The study of maturation and spawning of the oyster is part of a research program to investigate the summer mortalities of the oysters, Crassostrea gigas in Marennes-Oléron Bay. Four maturity stages were simultaneously obtained by diet and thermal conditioning (immature, low maturation, mature and post-spawning stages). Measurements of clearance, filtration, absorption and respiration rates allowed a calculation of the scope for growth and hence an estimation of the oyster's energetic budget at various maturity stages. Male and female oysters had similar physiological responses. The filtration rate ranged from 2.4 to 2.6 1.h(-1) at the early stages of maturation and decreased to 1.8 1.h.' during the maturity stage. Growth rate resulting from gonad...
Tipo: Text Palavras-chave: Huître; Crassostrea gigas; Conditionnement; Maturation; Filtration; Absorption; Respiration; Oyster; Crassostrea gigas; Conditioning; Maturation; Filtration; Absorption and respiration rates.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/publication-3090.pdf
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Effect of orgnotin compounds (TBT) used in antifouling paints on cultured marine molluscs - a literature study ArchiMer
Heral, Maurice; Alzieu, Claude; Deslous-paoli, Jean-marc.
Organotin salts, used as antifouling substances have a deleterious effect on the environment particularly on molluscs and especially on the Japanese oyster Crassostrea gigas. A synthesis of the effects of tributyltin (TBT) on cultured molluscs reveals the following effects: 1) acute and chronic toxicity to the adults; 2) accumulation in the flesh 01 the adults; 3) perturbation 01 reproduction particularly fertility, mortality 01 offspring and decrease in larval growth rate; 4) decrease in the growth rate 01 juveniles and thickening 01 the shell; and 5) modification 01 the physiology al sublethal levels.
Tipo: Text Palavras-chave: TBT; Crassostrea gigas; Oyster; Marine molluscs; Organotin.
Ano: 1989 URL: http://archimer.ifremer.fr/doc/1989/publication-3016.pdf
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Effect of sediment nearness on the metabolic enzyme activity and energy state of the oyster Crassostrea gigas ArchiMer
Le Moullac, Gilles; Fleury, Pierre-gildas; Le Coz, Jean-rene; Moal, Jeanne; Samain, Jean-francois.
This study was designed to assess in situ the effects of sediment nearness on Crassostrea gigas metabolism. One year-old oysters were reared for 5 months (April-August) in plastic bags on metallic frames at 10 and 60 cm off the sediment at the beginning of April. The management of the energetic resources (storage, consumption) was estimated with respect to biochemical changes of proteins, lipids and carbohydrates. In order to evaluate the effect of the sediment nearness at cellular level, the metabolic rate was evaluated by assessing pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK) enzyme activities and electron transport system (ETS) activity. The metabolic pathways were assessed by measuring ATP and intermediary metabolites such as...
Tipo: Text Palavras-chave: Aspartate; Succinate; Alanine; ETS; PEPCK; PK; Carbohydrate; Lipids; Proteins; Sediment; Oyster; Aspartate; Succinate; Alanine; ETS; PEPCK; PK; Glucides; Lipides; Proteines; Sédiment; Huître.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2803.pdf
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Effects of Alexandrium minutum exposure on nutrition-related processes and reproductive output in oysters Crassostrea gigas ArchiMer
Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Moal, Jeanne; Suquet, Marc; Gueguen, Marielle; Sunila, Inke; Soudant, Philippe.
This study assessed the effects of an artificial bloom of the toxin-producing dinoflagellate, Alexandrium minutum, upon nutrition related processes and reproductive output of the Pacific oyster, Crassostrea gigas. Oysters were exposed to A. minutum, Paralytic Shellfish Toxins (PST) producer and compared to a control batch of oysters fed Isochrysis galbana clone Tahitian (T.Iso). The experiment was performed in June 2008, when oysters were found ripe. Several physiological variables of oysters, such as PSTs accumulation, digestive gland and histological observations as well as spermatozoa quality, were measured at the end of the exposure. Results indicate that the digestive gland was greatly impacted upon A. minutum exposure. Monoacylglycerol and...
Tipo: Text Palavras-chave: Harmful algae; Alexandrium minutum; Oyster; Nutrition-related processes; Reproductive output.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00008/11927/8653.pdf
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Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts ArchiMer
Akcha, Farida; Barranger, Audrey; Bachere, Evelyne; Berthelin, C. Heude; Piquemal, D.; Alonso, P.; Sallan, R. Rondon; Dimastrogiovanni, G.; Porte, C.; Menard, Dominique; Szczybelski, A.; Benabdelmouna, Abdellah; Auffret, M.; Rouxel, Julien; Burgeot, Thierry.
Genitors of the Pacific oyster Crassostrea gigas were submitted during gametogenesis to a short pulse exposure to the herbicide diuron at a realistic environmental concentration. Histological analysis showed no effect of diuron on gametogenesis course, sex ratio and reproductive effort. A non-significant increase in testosterone and progesterone levels was observed in genitors exposed to the herbicide. At cell level, diuron exposure was shown to modulate the phagocytic activity of circulating hemocytes. The results of a transcriptional analysis showed that diuron affected the expression of genes belonging to functions known to play a major role during oyster gametogenesis such as gene transcription regulation, DNA replication and repair, DNA methylation...
Tipo: Text Palavras-chave: Oyster; Pesticide; Reproduction; Histology; Gene expression; RT-qPCR; Steroid; Immunity.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00309/41983/83232.pdf
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Effects of dissolved mercury on embryogenesis, survival, growth and metamorphosis of Crassostrea gigas oyster larvae ArchiMer
Beiras, Ricardo; His, Edouard.
The effects of mercury (Hg) concentrations ranging from 0 (control) to 1024 µg 1-1 upon embryogenesis, survival, growth and metamorphosis of Crassostrea gigas (Thunberg) oyster larvae were investigated. Embryogenesis was abnormal in 50 % of the individuals at 11 µg 1-1 . The 48 h LD50 for D·shaped, umbonate and pediveliger larvae were 33, 115 and 200 µg 1-1 respectively. The increase in LD50 was partially explained by the larval weight increase, although weight-specific tolerance to Hg was higher in smaller larvae. Growth, the most sensitive physiological process studied, was significantly retarded at 4 µg 1-1. The metamorphosis rate was significantly reduced when competent pediveligers were exposed to 64 µg 1-1 for 48 h prior to the addition of the...
Tipo: Text Palavras-chave: Mercury; Oyster; Larvae; Embryo; Bioassay; Ecotoxicology; Crassostrea gigas.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00037/14867/12191.pdf
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Effects of temperature and salinity on the survival of Bonamia ostreae parasite infecting falt oyster Ostrea edulis in sea water ArchiMer
Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could be demonstrated between the development of the disease and environmental parameters such as temperature or salinity. The parasite life cycle, including its survival outside the host is not completely known. Nevertheless, the infection can be directly transmitted by cohabitation between infected and non infected oysters suggesting that the parasite does not need intermediate host to complete its cycle. In the present study, the impact of temperature and salinity on the survival of purified parasites...
Tipo: Text Palavras-chave: Effects; Temperarature; Salinity; Bonamia ostreae; Parasite; Pathology; Flat oyster; Ostrea edulis; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3375.pdf
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Endurcissement du naissain d’huîtres creuses, Crassostrea gigas par le niveau d’exondation ou la densité d’élevage : récapitulatif d’essais conduits entre 2004 et 2007 en rivière d’Auray (56), pour réduire les mortalités estivales ArchiMer
Mazurie, Joseph; Bouget, Jean-francois; Langlade, Aime; Claude, Serge.
This report summarizes a list of husbandry experiments conducted between 2004 and 2007 so as to reduce the summer mortalities of the oyster Crassostrea gigas by farming practices. The Japanese technique so‐called “hardening”, based on nursing the young oysters (less than 1 year‐old) in intertidal areas with high air exposure, is tested and adapted. Significant improvements of survival rates (e.g. 60% survival instead of 40% survival rate) have been obtained by transferring the spat only a few month (between march and june‐july) on “high” inter‐tidal farming plots (up to 40% of mean air exposure time). In such conditions, growth is transiently reduced, but a compensatory growth seems to happen when oysters are transferred back on low concessions. Another...
Tipo: Text Palavras-chave: Huître; Crassostrea gigas; Mortalité; Endurcissement; Exondation; Densité; Oyster; Crassostrea gigas; Mortality; Hardening; Air exposure; Density.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00042/15350/12705.pdf
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Environment and immunomodulation : effects of the soluble fraction of Norwegian heavy fuel on the immune system of Pacifc oyster, Crassostrea gigas ArchiMer
Bado-nilles, Anne; Auffret, Michel; Quentel, Claire; Le Floch, Stéphane; Arzel, Jacqueline; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Hélène.
Estuaries and coastal areas are often subjected to much pollution, including pesticides and polycyclic aromatic hydrocarbons (PAHs) produced by anthropic activities. Bay of Marennes-Oléron is an estuarine coastal zone in which there is a high level of aquacultural production and this has adverse effects in aquatic organisms. In fact, PAHs, which are widely spread throughout aquatic ecosystems, have a low polarity and so there is a clear tendency to bioaccumulate in marine organisms (1). The harmful effects of such contaminants on the organism's immune system, leading to a decreased resistance to pathogens, is a relatively new area of research and it still poorly understood. The aim of the present work was to detect the impact in vivo of PAHs on the immune...
Tipo: Text Palavras-chave: PaHs; Contamination; Crassostrea gigas; Oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3428.pdf
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Epidémiologie de Bonamia ostreae et Marteilia refringens en Bretagne en 1984 et 1985 : situation et évolution ArchiMer
Tige, Gilbert; De Kergariou, Gabriel; Cochennec, Nathalie; Rabouin, Marie-agnès.
A systematic surveillance progam conducted since 1980 along the coasts of Britanny has followed the evolution and epidemiological state of the natural O. eulis oyster beds and seed areas in the intertidal zones and deep water. Presently, Marteilia refringens exists endemically in Brittany estuaries ; the rate and degree of infestation varies from year to year depending on the hydrological conditions and the geographical region. In no case, however, is the return of flat oyster culture envisoned. Bonamia ostreae is present not only in the estuaries but also in the deep-water rearing areas (Baie de Quiberon, Cancale, Baie de St Brieuc). The infestation increases with age, but also depends on the quantity of the stock and the culture practises. In particular,...
Tipo: Text Palavras-chave: Huître plate; Ostrea edulis; Maladie; Bonamia ostreae; Marteilia refringens; Bretagne; Oyster; Ostrea edulis; Diseases; Bonamia ostreae; Marteilia refringens.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/00022/13299/10353.pdf
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